Dr. Ruchita Selot
Post-doctoral fellow

Dr. Ruchita Selot

Post-doctoral fellow

3rd Floor, Narayana Nethralaya, Narayana Health City

# 258/A, Bommasandra, Hosur Road,

Bangalore, 560099 INDIA.


Contact Details
Email ruchita.selot@narayananethralaya.com
Phone +91-80-66660712

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Dr. Ruchita Selot has completed her PhD in Biotechnology from Mysore, India, She is currently working as Post doctoral fellow in Grow Research Laboratory, Her research expertise spans on AAV mediated gene therapy module development for ocular disorders and muscular dystrophies, with special focus on introduction of large genes by AAV Dual vector system. As an extension of gene therapy her work also involves Genome editing by CRISPR-Cas9 system to create disease models (in vitro and in vivo) and test the efficacy of AAV mediated transgene expression for restoration of normal tissue function.

ACADEMIC QUALIFICATION
PhD (Biotechnology)-Mysore [April 2010]
M.Sc. (Life Science)-Bhopal [June 2003]/CSIR-UGC NET [June-December 2003]
B.Sc. (Microbiology)-Nagpur [June 2001].

FIELD OF INTEREST
Epitopic domains in viruses and their involvement in host immune response induction
Peptide mediated host targeting by modification of drug/virus particle surface
Host-pathogen interaction and associated signalling pathways

RESEARCH EXPERIENCE

Post-Doctoral Scientist, GROW Lab, Narayana Nethralaya, Bangalore [Dec 2015-Current]

Project: 1) AAV Mediated Gene Therapy for treatment of Duchenne Muscular Dystrophy (PPMD funded)

2) Treatment of Retinitis Pigmentosa by AAV vector mediated gene delivery strategies

3) AAV mediated Gene Therapy module development for Duchenne Muscular Dystrophy amelioration

4) Generation of CRISPR-Cas9 knock out phenotypes to study ocular dysfunctions

5) Effect of multi-drug treatment(s) on corneal wound healing responses

Post-Doctoral Fellow, IIT Kanpur [Dec 2014-June 2015 Term completion]

Project: 1) Cloning of Laforin (LPME) and Malin (NHLRC1) genes in AAV for in vitro/in vivo studies

2) Evaluation of pooled IVIG interaction with AAVrh.10-WT vs. AAVrh.10-S671A mutant virus (in vitro)

Post-Doctoral Fellow, CSCR (CMC), Vellore [Dec 2011-June 2014]

Project: 1) Design and generation of AAV single/multiple mutant vector(s) to understand the role of viral capsid

structure and epitope interaction in host immune response elicitation

2) Screening and in vitro/in vivo validation of mutant vectors by Nab assay and IFN-Y ELISpot assay

3) In vitro and in vivo (mice) expression studies for AAVrh.10 mutant vectors

JRF/SRF (DBT) CSR&TI (CSB) Mysore [Aug 2004-July 2007]

Project: Identification and molecular characterization of antiviral BmNox protein from Bombyx mori.

GRANTS/AWARDS

International Travel Grant (ARVO, USA)-2018
International Travel Grant (Indian Council of Medical Research, India)-2015
International Travel Grant (Department of Biotechnology, India)-2015.

Abstracts/Posters 

1. Abstract entitled ‘Anti-BmNPV Activity and Differential Expression of BmNOX in Selected Strains of Silkworm Bombyx Morii accepted at 6th International Symposium on
Molecular Insect Science, Amsterdam, Netherlands 2011
2) Abstract entitled ‘Targeted bio-engineering of Specific Amino Acid Residues on Adeno Associated Virus Serotype rh.10 Capsid improves its gene delivery in vitro and in vivo’ accepted at 16th Annual ASGCT Meeting at Salt Lake City, Utah, USA 2013
3) Abstract entitled ‘Successful gene transfer in passively immunized mice with immunologically- inert AAVrh.10 vectors’ accepted at 18th Annual ASGCT Meeting at New Orleans, USA 2015
4) Poster entitled ‘Effect of Cyclosporine-A on injury-mediated EMT in corneal epithelial cells’ presented at Indo-Australian Conference on Role of EMT, 2017 at IISc, Bangalore
5) Work entitled ‘Increased expression of Retinoic acid-related Orphan Receptor gamma transcription factor and its products in keratoconus patients’ for oral presentation at ARVO, 2018 at Hawaii, USA [ARVO Travel Grant Awardee 2018]

List of Publications
1) Selot R., Kumar V., Shukla S., Kumar C., Brahmaraju M., Dandin S.B., Laloraya M. and Pradeep G. (2007) Identification of A Soluble NADPH Oxidoreductase (BmNox) with Antiviral Activities in the gut juice of Bombyx mori. Biosc. Biotech. Biochem. 71(1), 200-205
2) Selot R., Kumar V., Sekhar S. and Pradeep G. (2009) Molecular characterization and expression analysis of BmNox in two strains of Bombyx mori with contrasting viral resistance phenotype. Archives Insect Biochem. Physiol. 73(3): 163-175
3) Gabriel N., Hareendran S., Sen D., Gadkari R.A., Sudha G., Selot R., Hussain M., Dhaksnamoorthy R., Samuel R., Srinivasan N., Shrivastava A., and Jayandharan G.R. (2013) Bioengineering of AAV2 Capsid at specific Serine, Threonine or Lysine residues improves its transduction efficiency in vitro and in vivo. Human Gene Therapy Methods 1:1-14
4) Sen D., Gadkari R.A., Sudha G., Gabriel N., Sathish Y., Selot R., Samuel R., Rajalingam S., Ramya V. , Nair S.C., Srinivasan N., Srivastava A., Jayandharan. G. R. (2013) Targeted modifications in adeno-associated virus (AAV) serotype -8 capsid improves its hepatic gene transfer efficiency in vivo. Human Gene Therapy Methods 02/2013
5) Selot R.S., Hareendran S. and Jayandharan G.R. (2014) Developing immunologically inert adeno-associated virus (AAV) vectors for gene therapy: possibilities and limitations. Current Pharmaceutical Biotechnology 03 [Cited in Nature Reviews Dec 2018]
6) Selot R., Marepally S., Vemula P.K., Jayandharan G.R. (2015) Nanoparticle coated viral vectors for gene therapy. Current Biotechnology [Invited Review]
7) Selot R. et al. (2017) Optimized AAV rh.10 vectors that partially evade neutralizing antibodies during hepatic gene transfer. Frontiers in Pharmacology, July 2017