Dr. Chitra Gopinath
Postdoctoral fellow

Dr. Chitra Gopinath

Postdoctoral fellow

3rd Floor, Narayana Nethralaya, Narayana Health City

# 258/A, Bommasandra, Hosur Road,

Bangalore, 560099 INDIA.


Contact Details
Email chitra.g@narayananethralaya.com
Phone +91-80-66660712

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Postdoctoral Researcher working towards gene therapy for inherited retinal dystrophies. Skilled in adeno-associated virus and lentivirus vector production, molecular biology, human genetics and counselling.Postdoctoral Researcher working towards gene therapy for inherited retinal dystrophies. Skilled in adeno-associated virus and lentivirus vector production, molecular biology, human genetics and counselling.

EDUCATION 

Ph.D - Biomedical Sciences                                                                                                                                                 June 2019

School of Bio Sciences and Technology

Vellore Institute of Technology, Vellore, India

 M.Sc - Biomedical Genetics                                                                                                                                                     June 2011 

School of Bio Sciences and Technology

Vellore Institute of Technology, Vellore, India

B.Sc - Biotechnology, Chemistry, Botany                                                                                                                          Mar 2009 

Mount Carmel College, Bangalore, India

RESEARCH EXPERIENCE

 Postdoctoral Fellow                                                                                                                                           Jan 2019 - Current

GROW Lab, Narayana Nethralaya Foundation, Bangalore, India

Involved in AAV production for gene therapy

Involved in genetic education for GenEye Clinic

 Junior Research Fellow (ICMR project)   July 2015 - Feb 2018

Research Associate-  Oct 2012 - June 2015

School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, India

Worked on a project - “Gene therapy for leukocyte adhesion deficiency Type 1 (LAD1) using lentiviral vectors”

Developed novel lentiviral vectors and tested them on LAD1 patient B cell lines and hematopoietic stem cells in vitro

Trained in vector construction, lentivirus production, CRISPR-Cas9 mediated genetic manipulation and Epstein Barr virus mediated immortalization of lymphocytes

Accrued experience in writing project proposal

Visiting Research Scholar  .  Sept 2016 - May 2017

Laboratory of Genetics – Verma lab, Salk Institute for Biological Studies, La Jolla, CA, USA

Designing and codon optimization of the E. scolopes reflectin gene for testing in HEK293 cell line

Carried out transfections and analysis of experiments using flow cytometer and fluorescence imaging

Visiting Research Scholar . Sept 2015Feb 2016

GROW Lab, Narayana Nethralaya Foundation, Bangalore, India

Construction of lentiviral vector carrying RB1 gene for  generation of stable RB1+ retinoblastoma cell line

Involved in cloning of ABCA4 gene in AAV split-gene technique for  Stargardt’s gene therapy 

TEACHING EXPERIENCE . 2013- 2018     

School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, India

Mentored undergraduate project students and provided training in molecular and cell biology techniques

Assisted faculty members in conducting and teaching laboratory courses

Instructed graduate students in research article and thesis writing

 JOB EXPERIENCE . June 2011- Sept 2012

Molecular Diagnostics and Ocular Genetics  Lab, Narayana Nethralaya, Bangalore, India

Performed DNA, RNA extraction and polymerase chain reactions for diagnostics

Screening for mutations in RPE65 gene in patient blood samples

Construction of genetic pedigree charts

Accrued experience in professional environment, organized workshops and conferences

PUBLICATIONS

 Gopinath C, Nathar TJ, Ghosh A, Hickstein DD and Nelson EJR. Contemporary animal models for human gene therapy. Current Gene Therapy, 2015; 15(6):531-540.

Gopinath C, Chodisetty S, Ghosh A and Nelson EJR. Efficiency of different fragment lengths of the ubiquitous chromatin opening element HNRPA2B1-CBX3 in driving human CD18 gene expression within self-inactivating lentiviral vectors for gene therapy applications. Gene, 2019; 710:265-272.

Verma A, Gopinath C, Nelson EJR, Shetty R, and Ghosh A. Gene therapy vectors for inherited ocular disorders – A revolutionary medicine. Indian Journal of Opthalmology, (In press)

Book Chapter: Gopinath C, Nathar TJ, Nelson EJR. Retroviral vectors in gene therapy in ‘Gene and Cell Therapy: Biology and Applications’, Springer-Nature; ISBN 978-981-13-0481-1.

HONORS AND AWARDS

Awarded Fulbright-Nehru doctoral research fellowship . 2016-2017

 CONFERENCE PRESENTATIONS AND CERTIFICATES

 Oral and poster presentation - Efficiency of ubiquitous chromatin opening element HNRPA2B1-CBX3 in driving human CD18 gene expression within SIN lentiviral vector for gene therapy at the NGBT conference, Bhubaneswar , Odisha  [2017]

Certificate for animal handling workshop conducted by School of Biosciences and Technology, Vellore Institute of Technology, Vellore. [2013]                                                                                                                                  

Poster presentation - Gene therapy for leukocyte adhesion deficiency using lentiviral vectors. CAMTech India Hackathon, Vellore Institute of Technology, Vellore. [2012]

Certificate of appreciation for being part of the organizing team of the Regenerative and Transplantation Medicine in Ophthalmology conference (RTMO), Narayana Nethralaya, Bangalore [2011]

Participated and volunteered in the International Society for Genetic Eye Diseases & Retinoblastoma Meeting (ISGEDR), Narayana Nethralaya, Bangalore  [2011]

The International Conference on Biotechnological Solutions for Environmental Sustainability, Vellore Institute of Technology, Vellore.   [2009]                                                                                                                                                                             

Community Development Program on Local Health Tradition- Study of medicinal plants and preparation of home remedies for common ailments, MCC & FRLHT, Bangalore   [2005]